Seahorse Instruments

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UC customers: $229/assay
DARTs/NonProfits: $306/assay
External/For Profit: $351/assay

For questions or to get started, please contact unit director Alexey Tomilov, PhD, at atomilov@ucdavis.edu.
Or call 530-902-1051

The Seahorse XF flux analyzer equipment is available for recharge use at the UC Davis. The 24-well Seahorse system provides researchers the opportunity to perform medium-throughput, real-time measurement of mitochondrial function and cellular bioenergetics in an expedited manner compared to traditional techniques. Seahorse XF Analyzers measure oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in a multi-well plate, interrogating key cellular functions such as mitochondrial respiration and glycolysis. XF Analyzers perform compound addition and mixing, label-free analytical detection, and automatic measurement of OCR and ECAR in real time (https://www.agilent.com/en/products/cell-analysis/seahorse-analyzers).

Seahorse XF-24 Analyzers measure OCR and ECAR of live cells in a 24-well plate format, Seahorse-XFe96 – in 96-well format. OCR and ECAR rates are key indicators of mitochondrial respiration and glycolysis and these measurements provide a systems-level view of cellular metabolic function in cultured cells. The description of the systems can be found: https://www.agilent.com/en/product/cell-analysis/real-time-cell-metabolic-analysis/xf-analyzers 

• Real-time Results- this integrated system reports metabolic rates in just minutes, without sample extraction or labeling.  Wave software controls the instrument and performs rate measurements on the fly for same day results.
• Live-Cell Responses – detect responses to substrates, inhibitors, and other compounds in real time via the 4-port injection system and automated mixing while maintaining physiologic temperature (37oC).
• Sensitive- analyze as few as 10,000 cells per well in the custom 24-well plate.  Cell number requirements vary by cell type; consult the Cell Reference Database for details. The 24-well microplate and system has capacity for larger and/or more metabolically active samples than the 96-well system. The 96-well system requires smaller cell number, and can accommodate more samples simultaneously.
  
• Compatible with a variety of samples due to the precision-controlled heating tray, which can maintain 16-42oC (12oC-20oC above ambient conditions).
• Analyze function of islet or other mobile samples with the Seahorse XF24 Islet Capture Plate
• Measure mitochondrial function with the Seahorse XF Cell Mito Stress Test
• Generate a metabolic phenotype within one hour with the Seahorse XF Cell Energy Phenotype Test
• Measure glycolytic rates in live cells with the Seahorse XF Glycolytic Rate Assay
• Quickly determine dependency of cellular energy production on mitochondrial substrates with the Seahorse XF Mito Fuel Flex Test
• Easily create assay protocols and analyze data.
• For Research Use Only. Not for use in diagnostic procedures.

1. Users provide following information at the beginning of the experiment: Name, PI, Department, Tel., Contact Email, and Recharge Number, and the Number of Experiments (cartridges) to be used.
   
2. No Radioactive, Acutely Toxic, Toxic, Corrosive, or Flammable materials are allowed in the Instrument
   
3. Users are responsible for purchasing their own supplies, buffers biosensors and assay plates as listed on the Cortopassi lab website: https://www.cortopassilab.com/octet-instrument.html
   
4. Users prepare all necessary reagents, pipet their experimental components onto their assay plates, and bring these to the facility. If User needs to prepare the assay plates and cartridges in the Cortopassi lab space we can make arrangements to facilitate Users upon User request that should be made in advance.
   
5. Instrument Operator login to the Instrument Controller Software and make sure the User can execute the experiment. Instrument Operator can help Users to load the cartridges into the Instrument.
   
6. 6. Users are required to be familiar with UC Davis Safety policies and training, adhere to UC Davis Safety rules, and be able to provide appropriate documentation. Users are required to have appropriate PPE while in the laboratory space; the facility have extra lab-coats and gloves to facilitate Users.
   
7. Users will be charged according to the Number of Experiments performed.